hplc purified oligonucleotides Search Results


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Hybridon Inc hplc-purified, glp grade oligonucleotides
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Nihon Gene Research Laboratories hplc-purified ssdna oligonucleotide
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GeNOsys Inc hplc purified odn
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Metabion International AG hplc-purified oligonucleotides
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GeneWorks hplc-purified oligonucleotide 5′-(atat g tacatat)-3′
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Midland Certified Reagent oligonucleotides reverse-phase hplc purified
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CureVac Inc hplc-purified rna oligonucleotides 5′-gcguugg-3′
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BIOligo Biotechnology hplc-purified rna oligonucleotides
a This panel depicts duplexes formed by random <t>RNA</t> sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and <t>oligonucleotides</t> are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).
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biomers.net gmbh hplc purified oligonucleotides
a This panel depicts duplexes formed by random <t>RNA</t> sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and <t>oligonucleotides</t> are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).
Hplc Purified Oligonucleotides, supplied by biomers.net gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microsynth ag hplc purified oligonucleotides
a This panel depicts duplexes formed by random <t>RNA</t> sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and <t>oligonucleotides</t> are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).
Hplc Purified Oligonucleotides, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation oligonucleotides hplc grade
a This panel depicts duplexes formed by random <t>RNA</t> sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and <t>oligonucleotides</t> are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).
Oligonucleotides Hplc Grade, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microsynth ag hplc-purified single-stranded synthetic dna oligonucleotides specifying a 79-bp mtdna amplicon and a 97 gapdh amplicon
a This panel depicts duplexes formed by random <t>RNA</t> sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and <t>oligonucleotides</t> are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).
Hplc Purified Single Stranded Synthetic Dna Oligonucleotides Specifying A 79 Bp Mtdna Amplicon And A 97 Gapdh Amplicon, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a This panel depicts duplexes formed by random RNA sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and oligonucleotides are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).

Journal: Communications Biology

Article Title: Harnessing noncanonical trans -cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics

doi: 10.1038/s42003-024-07000-z

Figure Lengend Snippet: a This panel depicts duplexes formed by random RNA sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and oligonucleotides are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).

Article Snippet: HPLC-purified RNA oligonucleotides and biotin- or fluorophore/quencher-labelled oligonucleotides were purchased from Bioligo Biotech Co., Ltd. (Shanghai, China) and Sangon Biotech Co., Ltd. (Shanghai, China), respectively.

Techniques: Activity Assay, Concentration Assay, Fluorescence, Two Tailed Test