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Image Search Results
Journal: Communications Biology
Article Title: Harnessing noncanonical trans -cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics
doi: 10.1038/s42003-024-07000-z
Figure Lengend Snippet: a This panel depicts duplexes formed by random RNA sequences (RS/RNA) of various lengths (30 nt, 25 nt, 20 nt, 18 nt) paired with complementary ssDNA or RNA. Duplexes and oligonucleotides are denoted using Roman numerals (I–XII). The secondary structure of RS30/RNA, is predicted with the RNAfold algorithm. b , c The effect of each duplex and oligonucleotide (I–XII) on activating the trans -cleavage activity of Cas12a ( b ) and Cas13a ( c ) was assessed. All duplexes and oligonucleotides were tested at a final concentration of 100 nM. Raw fluorescence intensities at 120 min are presented. Illustrations were created with BioRender.com (Wu, Q. (2024) BioRender.com/p33h705). a.u., arbitrary units. n = 3 biologically independent experiments. Error bars represent mean ± SEM. Statistical significance was determined using unpaired two-tailed Student’s t -tests: ns (not significant, p > 0.05); **( p < 0.01); ****( p < 0.0001).
Article Snippet: HPLC-purified
Techniques: Activity Assay, Concentration Assay, Fluorescence, Two Tailed Test